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FABRICATION OF A CUSTOMIZED MICROEXTRUSION 3D BIOPRINTER

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Patient-specific treatments are crucial, and bioprinting is a promising technology in this context. 3D bioprinting involves layer-by-layer fabrication of cell-laden constructs to form functional three-dimensional tissues. The material used, known as bioink, is a blend of biocompatible matrix materials, patient-derived cells, and growth factors. 3D bioprinting is becoming a key technology in tissue engineering, although creating tissue constructs with integrated functional vasculature is still a significant challenge.
In this work, a customized 3D bioprinting system was designed and fabricated. This system can create functional tissue constructs and cell-free scaffolds for implantation and in-vitro studies. The printing system was characterized, and a bioink blend was established for fabricating viable tissue constructs.

The custom-designed 3D bioprinting system uses layer-by-layer fused deposition modeling controlled by computer software to achieve complex 3D shapes. It can generate 3D cell-laden microporous tissue constructs and cell-free scaffolds using hydrogel-based bioinks. The system accommodates various bioink compositions with different viscosities, nozzle diameters, and syringe capacities. Alginate-gelatin-based bioink was used to manufacture 3D cell-laden tissue constructs. The mechanical and physical properties of the constructs printed with different bioink compositions were studied. Additionally, the efficacy of the printing system was evaluated by examining cellular viability and morphological changes in mouse myoblast-based skeletal muscle tissue constructs for a specific bioink composition.

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